华西医学

华西医学

大鼠脑震荡伤后热休克蛋白 70 及胶质纤维酸性蛋白表达比值与损伤时间关系的研究

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目的 联合应用热休克蛋白 70(heat shock protein70,HSP70)及胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)阳性细胞率及表达比值的变化规律推断脑震荡的损伤时间。 方法 建立大鼠脑震荡伤动物模型,取脑组织进行 HPS70、GFAP 免疫组织化学(免疫组化)染色,经显微图像分析,计算伤后不同时间点两种蛋白指标表达的阳性细胞率及比值变化,了解其与损伤时间的关系。 结果 当 HSP70 与 GFAP 阳性细胞率的比值明显升高(从 7.15 升至 11.73),且 HSP70 阳性细胞率亦显著升高(与对照组比较 P<0.05),但 GFAP 阳性细胞率无明显改变时(与对照组比较P>0.05),提示损伤时间在伤后 0.5~3 h;当二者的比值较大(>6.66),HSP70 与 GFAP 阳性细胞率均有明显升高时(与对照组比较P<0.05),可推断损伤时间在伤后 3~12 h;当二者的比值较小(<6.66),HSP70 与 GFAP 阳性细胞率仍高于正常值时(与对照组比较P<0.05),损伤时间大致在伤后 12 h 以后。 结论 通过分析两种指标在脑震荡伤后不同时间点的阳性细胞率及比值变化规律,可在一定程度上推断出损伤时间的大致范围,此方法有助于脑震荡损伤时间的推断。

Objectives To detect expressions of heat shock protein70 (HSP70) and glial fibrillary acidic protein (GFAP), and to estimate the post-injury interval after brain concussion via the ratios of percentage of HSP70/GFAP-positive cells. Methods We established a brain concussion model of rat. Tissue levels of HSP70 and GFAP were determined by immunohistochemical staining at different time points after injury. Finally, the relationship between the ratio of percentage of HSP70/GFAP-positive cells and the post-injury interval was measured. Results The ratio of percentage of positive cells (from 7.15 to 11.73) and the percentage of HSP70-positive cells (P<0.05, compared with control group) increased, and the percentage of GFAP-positive cells did not change remarkably (P<0.05, compared with control group), what indicated the post-injury interval was between 0.5h and 3h. High ratio (>6.66) and high percentage of HSP70, GFAP-positive cells (P<0.05, compared with control group) indicated the post-injury interval was between 3h and 12h. A low ratio (<6.66) and high percentage of HSP70, GFAP-positive cells (P<0.05, compared with control group) suggested that the post-injury interval was later than 12h. Conclusion Via analyzing the variation rule of the ratio of percentage positive cells after brain concussion in this study, this new idea benefits estimating the post-injury intervals through a combination of different markers.

关键词: 脑震荡; 损伤时间推断; 热休克蛋白 70; 胶质纤维酸性蛋白; 阳性细胞率比值

Key words: Brain concussion; Post-injury interval; Heat shock protein70; Glial fibrillary acidic protein; The ratio of percentage of positive cells

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