华西医学

华西医学

白细胞介素-6 对子宫颈癌细胞株 C-33A 增殖影响的研究

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目的探讨白细胞介素-6(interleukin-6,IL-6)在子宫颈癌中的作用。方法将培养后的子宫颈癌细胞 C-33A 分为 IL-6 组和对照组,IL-6 组使用 IL-6(50 ng/mL)刺激,对照组不加入 IL-6,利用 MTT 比色法检测细胞增殖,划痕实验检测细胞迁移,实时荧光定量聚合酶链反应技术检测上皮钙黏着蛋白(epithelial-cadherin,E-Cad)、神经钙黏着蛋白(neural-cadherin,N-Cad)、波形蛋白(vimentin)、转录因子 Snail1(transcription factors-snail1,TFs-SNAIL1)在 mRNA 水平上的表达,蛋白质印迹法检测 E-Cad、N-Cad、Vimentin、TFs-SNAIL1 蛋白水平的表达。结果与对照组比较,IL-6 组子宫颈癌细胞株 C-33A 增殖活性更高(12 h:0.388±0.025 vs. 0.597±0.057;24 h:0.547±0.021 vs. 0.798±0.036;48 h:0.745±0.056 vs. 1.296±0.122;72 h:1.074±0.053 vs. 1.805±0.113;P<0.05),迁移能力更强(12 h:1.057±0.029vs. 1.200±0.045;24 h:1.189±0.036 vs. 1.428±0.181;48 h:1.273±0.059 vs. 1.569±0.143;72 h:1.409±0.047 vs. 1.623±0.170;P<0.05),E-Cad mRNA 及蛋白的表达更低(1.012±0.098vs. 0.483±0.171,P<0.01;1.032±0.015vs. 0.395±0.119,P<0.01),N-Cad mRNA 及蛋白表达更高(1.054±0.106vs. 1.465±0.230,P<0.01;1.040±0.043vs. 1.605±0.128,P<0.01),vimentin mRNA 及蛋白表达更高(1.050±0.083vs. 1.340±0.099,P<0.05;1.043±0.062vs. 1.430±0.077,P<0.05),TFs-SNAIL1 mRNA 及蛋白表达更高(1.058±0.176vs. 1.510±0.229,P<0.01;1.022±0.015vs. 1.470±0.139,P<0.01)。结论IL-6 可能促进子宫颈癌细胞株 C-33A 的增殖、迁移和上皮-间质转化。

ObjectiveTo explore the role of interleukin-6 (IL-6) in cervical cancer cell C-33A.MethodsThe cervical cancer cells C-33A were divided into the IL-6 group and the control group after culture. The IL-6 group were treated with 50 ng/mL of recombinant IL-6 protein, and the control group were without IL-6. Then cell viability and cell migration were detected by MTT assay and wound-healing assay, respectively. The mRNA and protein expressions of epithelial-cadherin (E-Cad), neural-cadherin (N-Cad), vimentin and transcription factors-snail1 (TFs-SNAIL1) were analyzed by real time quantitative polymerase chain reaction and Western blotting, respectively.ResultsCompared with the control group, in the IL-6 group the proliferation of cervical cancer cells C-33A was promoted (12 h: 0.388±0.025 vs. 0.597±0.057; 24 h: 0.547±0.021 vs. 0.798±0.036; 48 h: 0.745±0.056 vs. 1.296±0.122; 72 h: 1.074±0.053 vs. 1.805±0.113; P<0.05), and the relative migration ability of cervical cancer cell was promoted (12 h: 1.057±0.029vs. 1.200±0.045; 24 h: 1.189±0.036 vs. 1.428±0.181; 48 h: 1.273±0.059 vs. 1.569±0.143; 72 h: 1.409±0.047 vs. 1.623±0.170; P<0.05); meanwhile, compared with the control group, in the IL-6 group, the expression of E-Cad mRNA (1.012±0.098vs. 0.483±0.171, P<0.01) and E-Cad protein (1.032±0.015vs. 0.395±0.119; P<0.01) decreased, the expression of N-Cad mRNA (1.054±0.106vs. 1.465±0.230, P<0.01) and N-Cad protein (1.040±0.043vs. 1.605±0.128, P<0.01) increased, the expression of vimentin mRNA (1.050±0.083vs. 1.340±0.099, P<0.05) and vimentin protein (1.043±0.062vs. 1.430±0.077, P<0.05) increased, and the expression of TFs-SNAIL1 mRNA (1.058±0.176vs. 1.510±0.229, P<0.01) and Fs-SNAIL1 protein (1.022±0.015vs. 1.470±0.139, P<0.01) increased.ConclusionIL-6 may promote the proliferation, migration, and epithelial-mesenchymal transition of cervical cancer cell C-33A.

关键词: 子宫颈癌; 白细胞介素-6; 细胞上皮-间质转换

Key words: Cervical cancer; Interleukin-6; Epithelial-mesenchymal transition

引用本文: 唐林, 王琪琳, 王平. 白细胞介素-6 对子宫颈癌细胞株 C-33A 增殖影响的研究. 华西医学, 2018, 33(4): 423-426. doi: 10.7507/1002-0179.201803148 复制

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