华西医学

华西医学

干雾化过氧化氢对急救车内环境表面微生物杀灭效果的研究

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目的研究干雾化过氧化氢用于急救车内表面的消毒效果。方法2018 年 10 月—12 月用模拟现场试验和现场试验进行研究,模拟现场试验将嗜热脂肪杆菌(ATCC12980)芽孢菌片置于急救车厢内 6 处,使用 0.38~0.72 g/m3的干雾化过氧化氢消毒 60 min 后,培养观察是否长菌;现场试验为干雾化过氧化氢消毒前后使用物表测试平板分别进行急救车车厢内物表微生物采样,培养后计数菌落。结果置于无遮挡物体表面的菌片阴性率为 100%(20/20),有遮挡的表面菌片阴性率为 10%(1/10);干雾化过氧化氢消毒前后,物体表面菌落总数杀灭对数值均>1.00(26/26)。结论干雾化过氧化氢消毒自动化程度较高,对急救车内无遮挡物体表面消毒效果良好,适用于急救车内环境表面消毒,但应避免遮挡物对其消毒效果的影响。

ObjectiveTo investigate the disinfection effect of dry-fogging hydrogen peroxide (DFHP) on ambulance inner surfaces.MethodsThis study was carried out using simulated field test and field test from October to December 2018. In the simulated field test, the carriers with Geobacillus stearothemopilus (ATCC12980) spores were placed in 6 places in the ambulance, and disinfected for 60 minutes with DFHP of 0.38–0.72 g/m3. The carriers were cultured for up to 7 days to observe whether the bacteria were eliminated. Before and after the DFHP disinfection, the microbial sampling of the surface in the ambulance was carried out, and the colonies were counted after the cultivation.ResultsThe eliminating rate of the bacteria carriers on the uncovered surface was 100% (20/20), and which of the covered surface was 10% (1/10). The pass rate of microbial sampling was 100% (26/26).ConclusionsThe DFHP had a significant decontamination effect on the ambulance inner uncovered surfaces. The DFHP equipment is automated and their disinfecting quality is consistent, therefore it is suitable for the disinfection of ambulance inner surfaces. But the limitation of disinfection effect on covered surfaces should be avoided.

关键词: 干雾化过氧化氢; 急救车; 环境物表; 消毒

Key words: Dry-fogging hydrogen peroxide; Ambulance; Environmental surface; Decontamination

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