华西医学

华西医学

生物载体硫交联聚乙烯亚胺的合成及递送小干扰RNA的实验研究

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目的 合成可生物降解的基因载体,并分析其生物毒性及转染率。 方法 低分子量聚乙烯亚胺(PEI)通过双硫键交联合成可降解的高分子量PEI衍生物(SS-PEI),通过红外光谱和核磁波谱分析技术分析其化学结构,采用细胞活力实验和检测大鼠肝肾功能指标分析其细胞和体内毒性,并转染羟基荧光素修饰的siRNA(FAM-siRNA)分析细胞转染率。 结果 红外波谱和核磁波谱分析可见酰胺键的特征波谱,噻唑蓝法和肝肾功能指标显示SS-PEI不同剂量组与对照组的差异均无统计学意义(P>0.05),SS-PEI/FAM-siRNA转染率为(76.0 ± 2.8)%。 结论 SS-PEI的合成可明显提高装载siRNA的效率,具有安全、高效等特点。

关键词: 载体; 聚乙烯亚胺; 合成; 小干扰RNA; 递送

引用本文: 张玉娜,夏伟,倪晶,王国玉,庄菊花. 生物载体硫交联聚乙烯亚胺的合成及递送小干扰RNA的实验研究. 华西医学, 2013, 28(1): 1-5. doi: 10.7507/1002-0179.20130001 复制

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